Table 2 Production of 3HP- and 2HB-based polymers
Monomer supplying genes | Entry | Precursor concentration (g L−1)a | Cell dry weight (g L−1) | Polymer production (g L-1) | Monomer composition (mol%) | Mw (× 105) | Mn (× 105) | Mw/Mn | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2HB | 3HB | 3HP | 4H2MB | 5HV | 6HHx | |||||||||
pct, alkK | 15 | 3HP 1.0 | 4H2MB 1.0 | 3.53 ± 0.23 | 0.18 ± 0.05 | – | – | 100 | – | – | – | 2.2 | 1.0 | 2.3 |
pct, alkK | 16 | 3HP 1.0 | 5HV 1.0 | 3.20 ± 0.07 | 0.19 ± 0.04 | – | – | 100 | – | – | – | 3.4 | 1.4 | 2.4 |
pct, alkK | 17 | 3HP 1.0 | 6HHx 1.0 | 3.06 ± 0.06 | 0.19 ± 0.01 | – | – | 100 | – | – | – | 2.7 | 1.2 | 1.6 |
pct, alkK | 18 | 2HB 2.5 | 3HB 2.5 | 3.18 ± 0.05 | 0.40 ± 0.04 | 32.5 | 67.5 | - | – | – | – | 2.8 | 0.9 | 3.0 |
pct, alkK | 19 | 2HB 2.5 | 3HP 1.0 | 2.61 ± 0.13 | 0.16 ± 0.02 | 4.2 | - | 95.8 | – | – | – | 1.9 | 1.2 | 1.6 |
pct, alkK | 20 | 2HB 2.5 | 4H2MB 1.0 | 2.46 ± 0.24 | ND | – | – | – | – | – | – | – | – | – |
pct, alkK | 21 | 2HB 2.5 | 5HV 1.0 | 2.31 ± 0.20 | ND | – | – | – | – | – | – | – | – | – |
pct, alkK | 22 | 2HB 2.5 | 6HHx 1.0 | 2.47 ± 0.03 | ND | – | – | – | – | – | – | – | – | – |