Table 1 Production of 3HB-based copolymers and various homopolymers with and without AlkK
Monomer supplying genes a | Entry | Precursor concentration (g L−1) b | Cell dry weight (g L−1) | Polymer production (g L1) | Monomer composition (mol%) | Mw (× 105) | Mn (× 105) | Mw/Mn | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
2HB | 3HB | 3HP | 4H2MB | 5HV | 6HHx | |||||||||
pct | 1 | 3HB 2.5 | 3HP 1.0 | 3.67 ± 0.07 | 0.49 ± 0.10 | – | 51.4 | 48.6 | – | – | – | 6.3 | 2.8 | 2.3 |
pct | 2 | 3HB 2.5 | 4H2MB 1.0 | 3.68 ± 0.06 | 0.48 ± 0.03 | – | 97.9 | – | 2.1 | – | – | 2.9 | 0.8 | 3.6 |
pct | 3 | 3HB 2.5 | 5HV 1.0 | 3.17 ± 0.05 | 0.32 ± 0.02 | – | 100 | – | – | – | – | 2.0 | 0.9 | 2.3 |
pct | 4 | 3HB 2.5 | 6HHx 1.0 | 3.10 ± 0.04 | 0.33 ± 0.03 | – | 100 | – | – | – | – | 1.7 | 0.7 | 2.5 |
pct, alkK | 5 | 3HB 2.5 | 3HP 1.0 | 3.70 ± 0.13 | 0.78 ± 0.13 | – | 55.5 | 44.5 | – | – | – | 4.6 | 2.3 | 2.0 |
pct, alkK | 6 | 3HB 2.5 | 4H2MB 1.0 | 3.32 ± 0.21 | 0.66 ± 0.03 | – | 97.1 | – | 2.9 | – | – | 1.9 | 0.9 | 2.1 |
pct, alkK | 7 | 3HB 2.5 | 5HV 1.0 | 2.81 ± 0.38 | 0.42 ± 0.06 | – | 95.3 | – | – | 4.7 | – | 1.1 | 0.5 | 2.2 |
pct, alkK | 8 | 3HB 2.5 | 6HHx 1.0 | 2.85 ± 0.08 | 0.34 ± 0.02 | – | 98.2 | – | – | – | 1.8 | 0.8 | 0.4 | 2.1 |
pct, alkK | 9 | – | 2HB 2.5 | 2.61 ± 0.10 | NDc | – | – | – | – | – | – | – | – | – |
pct, alkK | 10 | – | 3HB 2.5 | 3.61 ± 0.07 | 0.44 ± 0.05 | – | 100 | – | – | – | – | 2.0 | 1.1 | 1.8 |
pct, alkK | 11 | – | 3HP 1.0 | 3.03 ± 0.08 | 0.25 ± 0.02 | – | – | 100 | – | – | – | 3.2 | 1.7 | 1.8 |
pct, alkK | 12 | – | 4H2MB 1.0 | 3.37 ± 0.63 | ND | – | – | – | – | – | – | – | – | – |
pct, alkK | 13 | – | 5HV 1.0 | 2.62 ± 0.10 | ND | – | – | – | – | – | – | – | – | – |
pct, alkK | 14 | – | 6HHx 1.0 | 2.44 ± 0.09 | ND | – | – | – | – | – | – | – | – | – |