Table 1 Strains and plasmids in this study

S. pogona

 NRRL 30,141

S. pogona, wild-type strain, plasmid-free parental strain

This lab

 S. pogona-ΔSP_2854

S. pogona, SP_2854 deletion strain

This work

 S. pogona::SP_2854

S. pogona, SP_2854 over-expressed strain

This work

E. coli

 DH5α

Escherichia coli, plasmid-free, host for general cloning

This lab

Plasmids

 pOJ260

E. coli-cloning vector, containing pUC18 replicon, oriT, Apr^R

This lab

 pKCcas9dO

acc(3)IV, pSG5, tipA-Scocas9, j23119, vector for expression

Huang et al. [32]

 pOJ260-UA_{SP_2854}-apr-DA_{SP_2854}

pOJ260 containing homologous region flanking SP_2854, for SP_2854 deletion

This work

 pKCcas9-SP_2854

pOJ260 containing PermE and SP_2854, for SP_2854 overexpression

This work