Fig. 3
Effects of SP_2854 deletion and overexpression on strain growth, glucose consumption and mycelial morphology. A Growth curve analysis of wild-type S. pogona and the S. pogona-ΔSP_2854 and S. pogona::SP_2854 mutants in SFM. B Comparison of glucose utilization in wild-type S. pogona and the S. pogona-ΔSP_2854 and S. pogona::SP_2854 mutants in SFM. C Effects of SP_2854 deletion on the transcription levels of PTSGlc genes involved in glucose uptake. The cells of the different strains were inoculated into SFM and cultured at 30 °C for 4 days. Total RNA was then isolated and used for qRT–PCR assays. The control strain was wild-type S. pogona. 16S rRNA served as the normalization control. D Scanning electron micrographs of wild-type S. pogona and the S. pogona-ΔSP_2854 and S. pogona::SP_2854 mutants. Comparison of mycelial morphology between the mutants and wild-type S. pogona on the 2nd, 4th and 6th days