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Fig. 2 | Microbial Cell Factories

Fig. 2

From: A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona

Fig. 2

SP_2854 positively regulates butenyl-spinosyn production in S. pogona. A Comparison of butenyl-spinosyn production in wild-type S. pogona and the S. pogona-ΔSP_2854 and S. pogona::SP_2854 mutants via HPLC analysis. Eight-day fermentation liquid was pretreated for HPLC analysis. B Genetic organization of the bus cluster in S. pogona. C Effects of SP_2854 deletion and overexpression on the transcription level of the bus cluster. The cells of the different strains were inoculated into SFM and cultured at 30 °C for 4 days. Total RNA was then isolated and used for qRT–PCR assays. The control strain was wild-type S. pogona. 16S rRNA served as the normalization control

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Microbial Cell Factories

ISSN: 1475-2859

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