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Fig. 1 | Microbial Cell Factories

Fig. 1

From: A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona

Fig. 1

Deletion and overexpression of SP_2854. A Transcription levels of SP_2854 at different time points via qRT–PCR analysis. B Neighbour-joining (NJ) distance tree constructed using the amino acid sequence SP_2854 from actinomycetes using MEGA6. C Schematic diagrams of the S. pogona-ΔSP_2854 mutant. D Schematic diagrams of the S. pogona::SP_2854 mutant. E PCR identification of the S. pogona-ΔSP_2854 mutant. Wild-type S. pogona genomic DNA was used as the control. Lane M, DL5000 DNA marker; Lanes 1 and 2, S. pogona-ΔSP_2854 mutant; Lane 3, control. The tested transformant showed a 2.3-kb PCR amplicon, whereas the control showed no band in that location. F PCR identification of the S. pogona::SP_2854 mutant. Wild-type S. pogona genomic DNA was used as the control. Lane M, DL2000 DNA marker; Lanes 1–4, S. pogona::SP_2854 mutant; Lane 5, control. The tested transformant showed a 1.3-kb PCR amplicon, whereas the control showed no band that location. G Sequencing of the S. pogona-ΔSP_2854 mutant PCR amplicon

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Microbial Cell Factories

ISSN: 1475-2859

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