Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO2 and H2

Table 1 Strains and plasmids used in this study

	Important features	Source
Strain
C. kluyveri DSM 555	Wild-type	DSMZ
C. ljungdahlii DSM 13528	Wild-type	DSMZ
C. lju pIM Hex#15	Wild-type C. ljungdahlii carrying the pIM Hex#15 plasmid	This work
C. lju Hex#15^gInt	C. ljungdahlii genomic integration strain with the Hex#15 gene cluster cassette and ermC	This work
C. lju Hex#15^gInt ΔermC	C. ljungdahlii genomic integration strain with the Hex#15 gene cluster cassette, and the ermC gene removed using CRISPR/Cas9	This work
C. lju Hex#15^gInt Ccar1^gInt	C. ljungdahlii double genomic integration strain with the Hex#15 and Ccar1 gene cluster cassettes, and the ermC gene removed using CRISPR/Cas9	This work
Plasmid
pIM Hex#15	Butyryl-CoA/hexanoyl-CoA cluster from C. kluyveri, adhE from C. acetobutylicum (SI 1.3), plasmid backbone with oriT/traJ, catP, ermC, himar1, and repH (SI 1.2): 25.6 kb	This work
pCJRK ΔermC	cas9, gRNA with N20 sequence for ermC, homology arms, catP, and repH: 12.6 kb (SI 1.5)	This work
pIM Ccar1	Butyryl-CoA/hexanoyl-CoA cluster 1 from C. carboxidivorans (SI 1.4), plasmid backbone with oriT/traJ, catP, ermC, himar1, and repH (SI 1.2): 15.5 kb	This work

ISSN: 1475-2859