Fig. 5

Fermentation of C. lju Hex#15^gInt Ccar1^gInt at 30 °C in ATCC 1754 medium at 2-L scale with a continuous supply of 20% CO₂ and 80% H₂ as the carbon and energy sources. A–E Fermentation with pH control (pH 6.0). F–J Fermentation without initial pH control. A, F Fermentation course showing the formation of biomass and heterologous products over time. B, G The pH of the fermentation broth plotted over time. C, H Maximum product concentrations and optical densities. D, I Abundance of heterologous enzymes encoded by the Hex#15 construct. E, J Abundance of heterologous enzymes encoded by the Ccar1 construct