Fig. 3
Comparison of plasmid-carrying and genomic integration strains. Maximum product concentrations and optical densities achieved by C. lju pIM Hex#15 (A, B) and C. lju Hex#15gInt (D, E) in serum bottle cultivations (A, D) and 2-L fermentations (B, E) with ATCC 1754 medium continuously supplied with 20% CO2 and 80% H2 as the carbon and energy sources. Data are means ± SD (n = 3 biological replicates, data in B: n = 1). Enzyme concentrations as determined by LC–MS/MS from C. lju pIM Hex#15 (C) and C. lju Hex#15gInt (F) in the early logarithmic (OD600 ~ 0.3–0.5), late logarithmic (OD600 ~ 0.5–1.0) and stationary phases (OD600 > 1.0) in a 2-L fermentation