Table 2 Primers used in this study
Gene | Primer sequence | Enzyme site |
|---|---|---|
Tryptophan 2,3-dioxygenase | F: 5′-gcTCTAGAgcaggaggattacaaaatgtaccatcaggttaatgaa-3′ | XbaI |
R: 5′-aaggaaaaaaGCGGCCGCttaaacgtttttttcttctccc-3′ | NotI | |
4-Hydroxyphenylpyruvate dioxygenase | F: 5′-gcTCTAGAgcaggaggattacaaaatgtcaaaagaagtaaaatcagt-3′ | XbaI |
R: 5′-aaggaaaaaaGCGGCCGCttataacgttcctcttttttctt-3′ | NotI | |
Homogentisate 1,2-dioxygenase | F: 5′-gcTCTAGAgcaggaggattacaaaatgctgaaaggttttgaactaa-3′ | XbaI |
R: 5′-aaggaaaaaaAGCGGCCGCttactctacccaggacttat-3′ | NotI | |
3-Hydroxyanthranilate 3,4-dioxygenase | F: 5′-gcTCTAGAgcaggaggattacaaaatggcaatagcaaaaccgtt-3′ | XbaI |
R: 5′-aaggaaaaaaGCGGCCGCttatctttttagggcataccg-3′ | NotI | |
Phytanoyl-CoA dioxygenase | F: 5′-gcTCTAGAgcaggaggattacaaaatggatcactataaagaacaag-3′ | XbaI |
R: 5′-aaggaaaaaaGCGGCCGCttagacaacctgtcccc-3′ | NotI | |
Phytanoyl-CoA dioxygenase, hypothetical protein | F: 5′-gcTCTAGAgcaggaggattacaaaatggatcactataaagaacaag-3′ | XbaI |
R: 5′-aaggaaaaaaGCGGCCGCtcagcgttgaaaaacctcc-3′ | NotI | |
Putative dioxygenase | F: 5′-gcTCTAGAgcaggaggattacaaactacacgttatgaaaaggaaa-3′ | XbaI |
R: 5′-aaggaaaaaaGCGGCCGCttatccattcactttaatagtgt-3′ | NotI | |
Hypothetical protein | F: 5′-gcTCTAGAgcaggaggattacaaaatggcaacactgcagcc-3′ | XbaI |
R: 5′-aaggaaaaaaGCGGCCGCttaaccgaattttaccgatgt-3′ | NotI |