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Table 1 Strains and plasmids used in this study

From: Melanin biopolymer synthesis using a new melanogenic strain of Flavobacterium kingsejongi and a recombinant strain of Escherichia coli expressing 4-hydroxyphenylpyruvate dioxygenase from F. kingsejongi

Strains and plasmids

Relevant properties

Source or references

Strains

 E. coli TOP10

F− mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG

Invitrogen

 E. coli BL21(DE3)

F− ompT gal dcm lon hsdSB(rB−mB−) λ(DE3[lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)

NEB

 Flavobacterium kingsejongi

 

KCTC 42908T

 Streptomyces avermitilis

 

KCTC 9063T

Plasmids

 pUCM

Cloning vector modified from pUC19; constitutive lac promoter, Ap

Kim et al. 2010

 pUCM_TDO

Constitutively expressing putative tryptophan 2,3-dioxygenase from F. kingsejongi, Ap

This study

 pUCM_HPPD

Constitutively expressing putative 4-hydroxyphenylpyruvate dioxygenase from F. kingsejongi, Ap

This study

 pUCM_HGD

Constitutively expressing putative homogentisate 1,2-dioxygenase from F. kingsejongi, Ap

This study

 pUCM_HAAO

Constitutively expressing 3-hydroxyanthranilate 3,4-dioxygenase from F. kingsejongi, Ap

This study

 pUCM_PHYH

Constitutively expressing putative phytanoyl-CoA dioxygenase from F. kingsejongi, Ap

This study

 pUCM_PHYH_HypoP

Constitutively expressing putative phytanoyl-CoA dioxygenase, hypothetical protein (dioxygenase) from F. kingsejongi, Ap

This study

 pUCM_PutaD

Constitutively expressing putative dioxygenase from F. kingsejongi, Ap

This study

 pUCM_HypoP

Constitutively expressing putative hypothetical protein (dioxygenase) from F. kingsejongi, Ap

This study

 pET-21a (+)

f1 ori, T7 promoter, C-terminal His-tag sequence, AmpR

Novagen

 pET-21a (+)_HPPD

f1 ori, T7 promoter, inducible expression of His6-tagged HPPD, AmpR

This study