Fig. 6
Batch fermentation of melanin-producing F. kingsejongi in a 5-L bioreactor. A The kinetics of cell growth and the rates of glucose and tyrosine consumption were monitored by measuring optical density at 600 nm (OD600) and quantifying the concentrations of glucose and tyrosine present in the culture broth. OD600 is represented by filled circles (●), residual glucose concentration by green triangles (▲), and residual tyrosine concentration by red rectangles (■). B Supernatant was collected from the culture at set time intervals and visually inspected. C The kinetics of F. kingsejongi melanin production were monitored by quantifying the melanin present in the culture broth during batch fermentation. D RT-PCR (left) and qRT-PCR (right) analysis of the hpd gene encoding HPPD in F. kingsejongi cells collected at 5, 19, and 50 h of fermentation culture. The tuf gene was used as a reference