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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Melanin biopolymer synthesis using a new melanogenic strain of Flavobacterium kingsejongi and a recombinant strain of Escherichia coli expressing 4-hydroxyphenylpyruvate dioxygenase from F. kingsejongi

Fig. 5

Multialignment of amino acid sequences of five Flavobacterium HPPD and homology modelling of the five Flavobacterium HPPDs and R. pickettii HPPD. A Amino acid sequences of F. kingsejongi HPPD and the four phylogenically close Flavobacterium HPPDs were aligned. The grey background represents 100% homology identities of amino acids between the five HPPDs, the red background represents 80% homology identities, and the brown background represents 50% homology identities. Amino acid sequences braced in red brackets represent the active site for 4-HPP binding. The yellow background indicates the binding residues in the active site for HPPD-4-HPP complex. B 3D molecular docking models for the HPPD-4-HPP complexes in the active site region of the HPPDs of F. kingsejongi (1), F. microcysteis (2), F. endophyticum (3), F. noncentrifugens (4), Flavobacterium sp. BFFFF1 (5), and R. pickettii (6) are presented. A molecular atom stick model of the substrate 4-HPP is presented. Hydrophobic, hydrogen bonding, and ionic interactions are indicated as dashed grey, blue, and yellow lines, respectively. Cavity sizes of each active site of the protein–ligand models are presented

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Microbial Cell Factories

ISSN: 1475-2859

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