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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Melanin biopolymer synthesis using a new melanogenic strain of Flavobacterium kingsejongi and a recombinant strain of Escherichia coli expressing 4-hydroxyphenylpyruvate dioxygenase from F. kingsejongi

Fig. 4

In vitro activity of purified 6 × His-tagged HPPD and native HPPD in crude protein extract. A HPPD activity was investigated using 4-HPP as a substrate for purified 6 × His-tagged HPPD. Red and blue arrows indicate peaks corresponding to homogentisate (product) and 4-HPP (substrate), respectively. B Tyrosinase activity was investigated using tyrosine as a substrate for purified 6 × His-tagged HPPD. C HPPD activity was investigated using 4-HPP as a substrate for native HPPD in a crude protein extract. Red and blue arrows indicate peaks corresponding to homogentisate (product) and 4-HPP (substrate), respectively. D Tyrosinase activity was investigated using tyrosine as a substrate for native HPPD in a crude protein extract

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Microbial Cell Factories

ISSN: 1475-2859

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