Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Melanin biopolymer synthesis using a new melanogenic strain of Flavobacterium kingsejongi and a recombinant strain of Escherichia coli expressing 4-hydroxyphenylpyruvate dioxygenase from F. kingsejongi

Fig. 3

Heterologous expression of the eight putative proteins from F. kingsejongi involved in melanin synthesis in E. coli; organization of genes involving homogentisate pathway of F. kingsejongi. A E. coli cultures expressing eight F. kingsejongi candidate genes after 2 days of growth at 30 °C on LB agar plates supplemented with 1 g/L tyrosine. Plate sections are designated as follows: 1, tryptophan 2,3-dioxygenase; 2, 4-hydroxyphenylpyruvate dioxygenase (HPPD); 3, homogentisate 1,2-dioxygenase; 4, 3-hydroxyanthranilate 3,4-dioxygenase; 5, phytanoyl-CoA dioxygenase; 6, phytanoyl-CoA dioxygenase; 7, putative dioxygenase; 8, hypothetical protein; C, empty plasmid (negative control). B Time-course monitoring of culture broth of E. coli expressing the putative HPPD. C Organization of genes encoding homogentisate pathway enzymes in F. kingsejongi

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us