Fig. 5
From: Combining nucleotide variations and structure variations for improving astaxanthin biosynthesis
a Schematic view of the verification of 6 nonsynonymous mutations. The wild-type target genes (YER164W, YOL084W, YNL054W-B, YBR198C, YGR032W, and YIL105C) were individually knocked out by URA3 in strain YSA448, followed by transfer of a pRS413 plasmid carrying the six mutated genes. b Astaxanthin yields in verified strains. (Student’s t-test; NS, not significant; *P < 0.05, **P < 0.01). In b, error bars represent SD from three independent experiments