Table 1 Steady-state kinetic parameters of Copu9 and its variants calculated from the EnzChek™ pyrophosphate assay

(3.24 ± 0.03) × 10^–2

4.59 ± 0.17

7.067

(5.02 ± 0.26) × 10^–2

6.28 ± 1.37

7.995

(2.91 ± 0.07) × 10^–2

3.14 ± 0.45

9.252

(3.96 ± 0.08) × 10^–2

8.28 ± 0.60

4.782

(3.60 ± 0.05) × 10^–2

7.00 ± 0.37

5.135

(3.98 ± 0.07) × 10^–2

8.48 ± 0.51

4.692

(3.81 ± 0.10) × 10^–2

6.82 ± 0.77

5.584

(2.62 ± 0.07) × 10^–2

4.42 ± 0.59

5.940

(1.92 ± 0.05) × 10^–2

5.10 ± 0.54

3.755