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Table 1 Steady-state kinetic parameters of Copu9 and its variants calculated from the EnzChek™ pyrophosphate assay

From: Biotechnological potential and initial characterization of two novel sesquiterpene synthases from Basidiomycota Coniophora puteana for heterologous production of δ-cadinol

Copu9 kcat [s−1] Km [µM] kcat/Km (× 103) [s−1 M−1]
WT (3.24 ± 0.03) × 10–2 4.59 ± 0.17 7.067
T73C (5.02 ± 0.26) × 10–2 6.28 ± 1.37 7.995
C76V (2.91 ± 0.07) × 10–2 3.14 ± 0.45 9.252
T192N (3.96 ± 0.08) × 10–2 8.28 ± 0.60 4.782
G194A (3.60 ± 0.05) × 10–2 7.00 ± 0.37 5.135
C195V (3.98 ± 0.07) × 10–2 8.48 ± 0.51 4.692
P197C (3.81 ± 0.10) × 10–2 6.82 ± 0.77 5.584
N228A (2.62 ± 0.07) × 10–2 4.42 ± 0.59 5.940
S314N (1.92 ± 0.05) × 10–2 5.10 ± 0.54 3.755