Fig. 5

In vivo flux distribution of Synechocystis 6803 ∆eda during photomixotrophic growth on glucose and CO₂ determined by GC–MS based ¹³C metabolic flux analysis. Fluxes are normalized to the glucose uptake (100%, 0.429 mmol g⁻¹ h⁻¹). The thickness of the arrows denotes the amount of flux. The errors for the fluxes reflect standard deviations, estimated by Monte-Carlo simulation. The anabolic fluxes into biomass are shown as triangles. The complete flux data set is given in Additional file 1: Table S2, where also the 95% confidence intervals from the Monte-Carlo analysis are provided. The abbreviations for the metabolites are explained in the legend of Fig. 4. The flux estimation yielded an excellent quality of fit for the considered mass isotopomers of amino acids, sugars, and sugar derivatives (Additional file 1: Table S3). The variance-weighted sum of squared residuals (SSR) was 377 and within the expected range (342–434) of the chi-square test at 95% confidence level, confirming also statistical acceptability (Additional file 1: Fig. S8). n = 4