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Fig. 2 | Microbial Cell Factories

Fig. 2

From: One-pot biosynthesis of 7β-hydroxyandrost-4-ene-3,17-dione from phytosterols by cofactor regeneration system in engineered mycolicibacterium neoaurum

Fig. 2

Rational design of the mP450-BM3 to improve its activity to AD. A Docking of the ligand AD into the mP450-BM3 crystal structure. AD is shown in green, heme is shown in red. B Relative activity of mP450-BM3 (WT) and mutants (away from the heme) to AD. C Relative activity of mP450-BM3 (WT) and mutants (close to the heme) to AD. D Relative activity of mP450-BM3 (WT) and the mutant (S72W/V78L/A82L/T88S/A328G/A330W) to AD. All assays were performed in triplicate with three independent measurements. Standard deviations of the biological replicates are represented by error bars

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Microbial Cell Factories

ISSN: 1475-2859

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