Sex differences in rumen fermentation and microbiota of Tibetan goat

Table 6 Primer sequences of fatty acid-related genes

Gene	Primer (5′–3′)	Length	Annealing temperature (°C)	Accession number
β-actin	F:AGCCTTCCTTCCTGGGCATGGA	113 bp	60	NM_001009784.3
β-actin	R:GGACAGCACCGTGTTGGCGTAGA	113 bp	60	NM_001009784.3
DRA	F:TGTGGCGGCTTCCAGAATTT	167 bp	60	NM_001280717.1
DRA	R:CACAGGCTTGTTTGGGAGCA	167 bp	60	NM_001280717.1
MCT1	F:GGACTGTGTCATCTGGCAGC	134 bp	60	XM_004002335.4
MCT1	R:TGGGGTCCAACAAGGTCCAT	134 bp	60	XM_004002335.4
AE2	F:AAGATCCCTGAGAACGCCGA	152 bp	60	XM_027969012.1
AE2	R:AGCAGAAAGAGGAAGCGCAC	152 bp	60	XM_027969012.1
NHE2	F:TTCTTTGTCGTGGGGATCGG	180 bp	60	XM_027967037.1
NHE2	R:CGTGATTGCCATGATGCCTG	180 bp	60	XM_027967037.1
MCT4	F:ACGGCTCAGCCTTAGTAAACTTC	144 bp	60	NC_0402252.1
MCT4	R:AATGGAGTTGTGCGAGTTGGT	144 bp	60	NC_0402252.1
NHE1	F:GCTTCTTCGTGGTGTCCCTG	174 bp	60	XM_004005085.4
NHE1	R:CCATGATGCCTGACAGGTGG	174 bp	60	XM_004005085.4

ISSN: 1475-2859