Table 5 Primer sequences of microbiota
From: Sex differences in rumen fermentation and microbiota of Tibetan goat
Gene | Primer(5′–3′) | Length | Annealing temperature (℃) | Accession number |
|---|---|---|---|---|
Bacterium | F:CCTACGGGAGGCAGCAG | 181Â bp | 60 | * |
R:TTACCGCGGCTGCTGG | ||||
Rf | F:TATCTTAGTGGCGGACGGGT | 157Â bp | 60 | MT356193.1 |
R:TCTAATCAGACGCGAGCCCA | ||||
Fs | F:GATGAGCTTGCGTCCGATT | 110Â bp | 60 | EU606019.1 |
R:ATTCCCTACTGCTGCCTCC | ||||
Ra | F:GGGCTTAACCCCTGAACTGC | 114Â bp | 60 | X85098.1 |
R:TCGCCACTGATGTTCCTCCT | ||||
Ram | F:GGGGACAACACCTGGAAACG | 124Â bp | 60 | Y15992.1 |
R:CTTGGTAGGCCGTTACCCCA | ||||
Bf | F:CCTGACTAAGAAGCACCGGC | 107Â bp | 60 | U41167.1 |
R:GTAAAACCGCCTACGCTCCC | ||||
MB | F:TCTGTACGGGTTGTGAGAGCA | 106Â bp | 60 | KP752401.1 |
R:CGCGATTTCTCACATTGCGG |