Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production

Table 1 Performance metrics of freeze–thaw permeabilized cell catalysts in COS synthesis

	Soluble COS yield (8 h) %	Initial cell catalyst activity (1 h) U/g_CDW	Product titer (8 h) g/L	STY (8 h) g/L/h	TTN (8 h) G_product/g_CDW
pPOLY_2	83	315	27	3.4	10.3
pPOLY_1	60	279	24	3.0	9.0
pDUBI	78	221	26	3.3	9.9

Reaction mixture consisted of 200 mM sucrose, 65 mM glucose, 50 mM phosphate, 50 mM MES, pH 7.0, 2.6 g_CDW/L whole-cell catalyst

ISSN: 1475-2859