Fig. 6From: Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble productionOptimized conversion catalysed by freeze–thaw permeabilized pPOLY_2 whole-cell catalyst at 45 °C. A Conversions of the substrates sucrose and glucose to fructose and total soluble COS. B Production of single soluble COS species over time (DP 2–6). C Product composition after 6 h reaction timeBack to article page