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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production

Fig. 5

Different preparations of pPOLY_2 catalyst analyzed for COS synthesis: A cell-free extract, B non-permeabilized cells, C freeze-dried cells, D freeze–thaw treated cells. Reaction conditions: 200 mM sucrose, 65 mM glucose, 50 mM phosphate in 50 mM MES buffer, pH 7 at 30 °C, 2.6 gCDW/L reaction mixture

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Microbial Cell Factories

ISSN: 1475-2859

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