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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production

Fig. 2

Vector design for enzyme co-expression with two plasmids. A p15A and pBR322 medium copy ori, pUC19 high copy ori. Single plasmid co-expression: Combinatorial strategy (B pDUBI) with two expression cassettes oriented in opposite directions and polycistronic strategies (C pPOLY_1 and pPOLY_2). All plasmid maps are depicted in full in the Additional file 1: Fig. S3. Additionally, the plasmid maps and full sequences were deposited in the add gene database, accession numbers 179272 to 179276 (https://www.addgene.org)

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Microbial Cell Factories

ISSN: 1475-2859

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