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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Retro-protein XXA is a remarkable solubilizing fusion tag for inclusion bodies

Fig. 3

XXA exhibited its solubilizing effect at both high and low temperatures. a Most sumo-NbALFA fusion proteins were expressed in an insoluble form at 16Ā Ā°C. b The NbALFA was expressed as soluble at 16Ā Ā°C after fusion with XXA. c Most MBP-CUA63106 fusion proteins were expressed as insoluble at 25Ā Ā°C. d The CUA63106 was expressed as soluble at 25Ā Ā°C after fusion with XXA. M marker, U uninduced whole cells, I induced whole cells, S supernatant of the induced cells, P precipitate of the induced cells

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