Fig. 7

Production titers of: A Strains 135, 220 and 133, which were selected as the three best naringenin producers in the biosensor-driven screening (see Fig. 3B) and the six strains, Top10.x, bearing the nariningenin biosynthesis pathways which were predicted by the articial neural network (ANN) ensemble model to be the top candidates to yield optimized naringenin production titers (see Fig. 6C). n = 5 except for Top10.6 which has n = 1; B The two best performing strains, Top10.4 and Top10.5, compared to a reference strain (NarRef) expressing a non-optimized pathway driven by an identical constitutive sigma factor (σ) 70 promoter for each gene and compared to these strains grown on a culture medium that was supplied with glucose (yellow bars with single hatching) or a carbon-equimolar amount of glycerol (yellow bars with cross hatching). (Error bars: standard deviation; Outliers (filled red squares) are identified with Grubbs’ test for outliers [47], Statistical tests: ANOVA and Tukey for multiple comparison)