Fig. 8

Regulation of the expression of the Pc-yap1 gene by PcRsmA. A Semiquantitative RT-PCR using as template RNA extracted from mycelium grown for 36 h in submerged cultures (medium MPPY); primers were the same as indicated in the legend to Fig. 3. The left panel shows the intensity of the bands in an agarose gel loaded with the products of the RT-PCR reactions, and the right panel the densitometry analysis of the bands. The results were normalized with the bands of the constitutively expressed act gene, and the parental strain Wis54-1255 (lane W) was used as reference with a value for integrated optical density (IOD) of 100. R24: strain si-PcRsmA-24; R25: strain si-PcRsmA-25. B Northern blot with RNA extracted from mycelium grown on solid Power medium for 120 h; the probes used were a 458 bp fragment from the Pc-yap1 gene amplified by PCR with primers siYAP1-F and -R, and a 508 bp fragment from the act gene amplified by PCR with primers N-actA-1F and R (Additional file 12). At the right, the densitometry analysis was performed as described above, adjusting the IOD of strain Wis54-1255 to 1. (See “Materials and methods” section for details)