Fig. 5

EMSA of the PcAtf21 binding activity in the pcbAB-pcbC intergenic region and of the three bZIP transcription factors in a region of the penDE gene promoter. A The six probes (lanes 1 to 6) of the pcbAB-pcbC intergenic region (Fig. 4A) were tested for binding to the PcAtf21 transcription factor; a binding reaction with probe 5 and PcYap1 was used as positive control. B EMSA of the PcYap1, PcRsmA, and PcAtf21 binding activities with probe penDE-CRE123, whose sequence corresponds to a central region in the penDE gene promoter; probe norR4 was used as positive control for PcAtf21 binding (see text). C Sequences of the probes used for EMSA. Probe penDE-CRE123 contains a CRE site (highlighted in red) and two CRE-like sites upstream (highlighted in pink); the nucleotide changes at the CRE site in the probe penDE-CRE123-M1 are highlighted in blue