Fig. 10

Regulation of the expression of the brlA gene by PcYap1 and PcRsmA. A Northern blot with RNA extracted from mycelium grown on solid Power medium for 120 h; the probes used were a 573 bp fragment from the brlA gene amplified by PCR with primers N-brlA-1F and R, and a 508 bp fragment from the act gene amplified by PCR with primers N-actA-1F and R (Additional file 12). The densitometry analysis was performed as described in the legend to Fig. 8. Strain Q204L was used as a control for brlA gene transcriptional repression (see text). B EMSA of the PcYap1 and PcRsmA binding activities with the probe AP1-brlA, whose sequence corresponds to a region located between − 58 and − 90 bp upstream the ATG start codon of the brlA gene promoter, and which contains an AP1 site (highlighted in red); this site is mutated in the AP1-brlA-Mut probe