Table 2 Butyl acetate production using different E. coli strains
From: Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate
Strain | Genes expressed | Acetate esters (g/L) | By-products (g/L) | Glucose consumption (g/L) | Cell optical density (OD600) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Butanol biosynthesis | ATF1 | fdh | Promoter used | Butyl acetate | Ethyl acetate | Ethanol | Butanol | Acetate | Pyruvate | Formate | |||
YA1 | O | O | LlacO1 | 0 ± 0 | n.d. | 0.05 ± 0.01 | 1.8 ± 0.2 | 0.92 ± 0.08 | 1.6 ± 0.3 | 0 ± 0 | 9.7 ± 0.8 | 2.0 ± 0.2 | |
YA2 | O | O | O | LlacO1 | 0.39 ± 0.1 | n.d. | 0.05 ± 0.01 | 0.42 ± 0.05 | 1.02 ± 0.03 | 0.50 ± 0.08 | 0.38 ± 0.02 | 8.0 ± 0.4 | 0.87 ± 0.05 |
YA3 | O | O | LlacO1 | 1.4 ± 0.1 | n.d. | 0.12 ± 0.01 | 0.33 ± 0.02 | 1.28 ± 0.04 | 1.4 ± 0.2 | 0.59 ± 0.19 | 12 ± 0.4 | 1.3 ± 0.1 | |
YA5 | O | O | FRE | 1.5 ± 0.1 | n.d. | 0.18 ± 0.01 | 0.53 ± 0.04 | 1.42 ± 0.03 | 1.8 ± 0.2 | 0.63 ± 0.06 | 13 ± 0.1 | 1.5 ± 0.1 | |