Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate

Table 1 Strains and plasmids used in this study

	Relevant genotype	References
Strain
BW25113	rrnB_T14 ΔlacZ_WJ16 hsdR514 ΔaraBAD_AH33 ΔrhaBAD_LD78
XL1-blue	recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacI^qZΔM15 Tn10 (Tet^R)]	Agilent Technologies
JCL16	BW25113/F’ [traD36 proAB⁺ lacIqZΔM15 (Tet^R)]	[39]
JCL299	JCL16 ΔadhE ΔldhA ΔfrdBC Δpta	[39]
YA1	JCL299 transformed with pEL11, pIM8, pBA1	This work
YA2	JCL299 transformed with pEL11, pBA5, pBA1	This work
YA3	JCL299 transformed with pEL11, pBA5	This work
YA5	JCL299 transformed with pRW13, pYA2	This work
Plasmid
pMW1	P_LlacO1::ATF1; ColA ori; Kan^R	This work
pMW4	P_LlacO1::ATF1; ColE1 ori; Amp^R	This work
pBA1	P_LlacO1::fdh; pSC101 ori; Spec^R	This work
pBA3	P_LlacO1::ATF1; pSC101 ori; Spec^R	This work
pBA5	P_LlacO1::ter, ATF1; ColA ori; Kan^R	This work
pYA2	P_adhE::ter, ATF1; ColA ori; Kan^R	This work
pRW13	P_ack::atoB, adhE2, crt, hbd; ColE1 ori; Amp^R	[29]
pRW18	P_adhE::fdh; pSC101 ori; Cm^R	[29]
pRW22	P_adhE::ter; ColA ori; Kan^R	[29]
pEL11	P_LlacO1::atoB, adhE2, crt, hbd; ColE1 ori; Amp^R	[26]
pIM8	P_LlacO1::ter; ColA ori; Kan^R	[26]
pCS138	P_LlacO1::fdh; pSC101 ori; Cm^R	[26]

ISSN: 1475-2859