Fig. 2

Evaluating the effect of expressing ATF1 on different plasmid vectors for converting butanol to butyl acetate and butyl acetate toxicity to E. coli. A Product concentration in the culture broth and B cellular growth of E. coli JCL16 carrying ATF1 gene on different copy number plasmid vectors. The cultures were incubated at 37 °C anaerobically for 24 h with butanol supplemented. The origin of replication, ColE1, ColA and pSC101 represent the relative copy number high, medium and low, respectively. C Growth of E. coli culture after 24 h with different concentration of butyl acetate added to the culture. T-test was performed to the experimental data. A p value larger than 0.05 is labeled as n.s