Fig. 4From: Altering the fatty acid profile of Yarrowia lipolytica to mimic cocoa butter by genetic engineering of desaturasesPromoter exchange of native FAD2 gene. Strains were expressing the native FAD2 gene under the control of different promoters. The strains were either expressing the native YlOLE1 or RtOLE1 under the control of the native OLE1 promoter, respectively. Strains were cultivated for 96 h in LPU media, and the fatty acid profile was determined by FAME extraction. Lipid content represents g FAME per g cell dry weight. Displayed is the mean and standard error of n ≥ 4 replicatesBack to article page