Fig. 3
Promoter exchange of OLE1 homologs. The native OLE1 gene was exchanged for either RtOLE1 or AaOLE1, and the native promoter was exchanged for either pGAPDH or pRP30. A Strains were cultivated for 96 h in LPU media, and the fatty acid profile was determined by FAME extraction. Lipid content represents g FAME per g cell dry weight. Displayed is the mean and standard error of n ≥ 4 replicates. B Scheme of expected promoter activity of pRP30 and pGAPDH based on data from [24]. Dashed line marks the onset of lipid production due to nitrogen limitation. C Growth curves of strain KSYL07. Cells were cultivated in 96-well plates in LPU media with or without the addition of palmitoleic acid (C16:1) or oleic acid (C18:1) (500 mg/L in 1% Tween 20), and OD600 was measured every 30 min. The lines and shadows represent the average and standard deviation of quadruplicates, respectively