Table 3 List of strains and plasmids used for the production of 5-HTP and 5-HT
From: Construction of cell factory capable of efficiently converting l-tryptophan into 5-hydroxytryptamine
Name | Description | References |
|---|---|---|
Strains | ||
E. coli BL21(DE3) | Protein expression host | This lab |
E. coli BL21 (DE3)△tnaA | E. coli BL21(DE3)△tnaA | This study |
E. coli Trans 1T1 | Cloning host | This study |
E. coli BL21(DE3) △tnaA /BH4/SmTPH | E. coli BL21(DE3)△tnaA/pCDFDuet-BH4/pET-28a( +)-SmTPH | This study |
E. coli BL21(DE3) △tnaA/MH4/SmTPH | E. coli BL21(DE3)△tnaA/pCDFDuet-MH4/pET-28a( +)-SmTPH | This study |
E. coli BL21(DE3) △tnaA/HaDDC | E. coli BL21(DE3)△tnaA/pET-28a( +)-SmTPH | This study |
E. coli BL21(DE3) △tnaA/BH4/SmTPH/HaDDC | E. coli BL21(DE3)△tnaA/pCDFDuet-BH4/pET-28a( +)-SmTPH-HaDDC | This study |
E. coli BL21(DE3) △tnaA/BH4/HaDDC/SmTPH | E. coli BL21(DE3)△tnaA/pCDFDuet-BH4/pET-28a( +)- HaDDC-SmTPH | This study |
Plasmids | ||
pET-28a ( +) | ColE1 ori, KanR, E. coli expression vector | This lab |
pET-28a ( +)-SmTPH | pET-28a ( +); tryptophan hydroxylase gene from S.mansoni | This study |
pCDFDuet-1 | CloDF13 ori, SmR, E. coli expression vector | This lab |
pCDFDuet-BH4 | pCDFDuet-1; pterin-4-alpha-carbinolamine dehydratase (PCD), dihydropteridine reductase (DHPR), 6-pyruvate-tetrahydropterin synthase (PTPS), sepiapterin reductase (SPR), GTP cyclohydrolase I (GCHI) | [1] |
pCDFDuet-MH4 | pterin-4-alpha-carbinolamine dehydratase (PCD) and dihydropteridine reductase (DHPR) from human | [18] |
pET-28a ( +)-HaDDC | pET-28a ( +); dopa decarboxylase from H.axyridis | This study |
pET-28a ( +)-HaDDC-SmTPH | pET-28a ( +); dopa decarboxylase from H. axyridis; tryptophan hydroxylase gene from S. mansoni | This study |
pET-28a ( +)-SmTPH- HaDDC | pET-28a ( +); tryptophan hydroxylase gene from S. mansoni; dopa decarboxylase from H. axyridis | This study |
Primers | ||
HaDDC-F-BamHI | GGATCCGAATTCATGGAGGCG | This study |
HaDDC -R-HindIII | AAGCTTTTATTCACCCAGGATATC | This study |
HaDDC-F-EcoRI | GAATTCATGGAGGCGAACCAG | This study |
HaDDC-R-SalI | GTCGACTTATTCACCCAGGATATCGTCC | This study |