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Table 1 Comparison of GFPuv expression levels in various media (% of IPTG control)

From: T7-lac promoter vectors spontaneous derepression caused by plant-derived growth media may lead to serious expression problems: a systematic evaluation

Percent of GFPuv expression

Time (h)a

T0

T1

T2

T3

T4

T5

IPTGb control (control)

15.8

39.2

65.3

83

88.7

100

Soy peptone

12.3

15.1

27.2

54

63.9

94.4

M9 minimal medium

ND

ND

ND

ND

ND

ND

Yeast extract only

ND

ND

ND

ND

ND

ND

Malt extract

ND

ND

ND

ND

ND

ND

Tryptone peptone

ND

ND

ND

ND

ND

ND

Tryptose peptone

ND

ND

ND

ND

ND

ND

Gelatin peptone

ND

ND

ND

ND

ND

ND

Casein peptone

ND

ND

ND

ND

ND

ND

Proteose peptone

ND

ND

ND

ND

ND

ND

Peptobak

ND

ND

ND

ND

ND

ND

  1. ND not detectable using densitometry of stained SDS-PAGE gels
  2. aStarting from OD600  = 0.6–0.8
  3. bIPTG-induced control in tryptone peptone-based medium