Fig. 4
Expression leakage of RM.TthHB27I in E. coli. The cultures were carried out as for E. coli BL21(DE3) [pET21d(+)-gfpuv]. The bacterial cells were lysed and analysed on 7.5% SDS-PAGE. (Lane M) PageRuler™ Unstained Broad Range Protein Ladder (Thermo Scientific); (Lane K) purified recombinant RM.TthHB27I protein; (Lane T0) E. coli BL21(DE3) [pET21d(+)-tthHB27IRM] cells at OD600 = 0.6–0.8; (Lane T1) cells 1 h after the culture reached OD600 = 0.6–0.8; (Lane T2) 2 h; (Lane T3) 3 h; (Lane T4) 4 h; (Lane T5) 5 h; (land T6) 6 h; and (Lane TO/N) cells after overnight cultivation. The arrows indicate the position (127.7 kDa) at which RM.TthHB27I migrates in the gel. The red arrow indicates RM.TthHB27I in the samples containing the expressed tthHB27IRM gene