Table 1 List of plasmid backbones and Synechocystis strains used in this study
Construct | Comment | Antibiotics resistance | References |
|---|---|---|---|
pEEK | RSF 1010-based expression vector with ccdB flanked by cloning sites and the ribosome binding site RBS*, promoter Ptrc2O, and a C-terminal Strep-tag sequence | Km | [37] |
pEEK* | Same as pEEK but without ccdB, used as empty vector control | Km | Englund, E., not published |
pEEKN | Same as pEEK but with N-terminal Strep-tag sequence | Km | This study |
pEEK3 | Based on pEEC3 [38], with PtrcCore promoter, RiboJ and N-terminal Strep-tag sequence, but with Km resistance | Km | Englund, E., not published |
pEEK3C | Same as pEEK3 but with C-terminal Strep-tag sequence | Km | This study |
pEERM3 | Empty backbone based on pJ344 vector backbone for integration into neutral site 1 (slr1068) of Synechocystis 6803, carrying PnrsB promotor and RBS* | Cm | [39] |
p1573 | Same as pEERM3 but with homologous recombination regions to slr1573 | Cm | This study |
Strains | Comment | Antibiotics resistance | References |
|---|---|---|---|
EvC | Control strain of Synechocystis WT harboring pEEK* plasmid for Km resistance | Km | This study |
PcC | Petroselinum crispum PAL expressed from Ptrc RBS* in pEEK | Km | This study |
AtC | Arabidopsis thaliana PAL expressed from Ptrc RBS* in pEEK | Km | This study |
NpC | Nostoc punctiforme PAL expressed from Ptrc RBS* in pEEK | Km | This study |
AvC | Anabaena variabilis PAL expressed from Ptrc RBS* in pEEK | Km | This study |
TsC | Treponema socranskii PAL expressed from Ptrc RBS* in pEEK | Km | This study |
TsC-H87L | Treponema socranskii PAL with mutation H87L expressed from Ptrc RBS* in pEEK | Km | This study |
PcN | Petroselinum crispum PAL expressed from Ptrc RBS* in pEEKN | Km | This study |
AtN | Arabidopsis thaliana PAL expressed from Ptrc RBS* in pEEKN | Km | This study |
NpN | Nostoc punctiforme PAL expressed from Ptrc RBS* in pEEKN | Km | This study |
AvN | Anabaena variabilis PAL expressed from Ptrc RBS* in pEEKN | Km | This study |
TsN | Treponema socranskii PAL expressed from Ptrc RBS* in pEEKN | Km | This study |
TsN-H87L | Treponema socranskii PAL with mutation H87L expressed from Ptrc RBS* in pEEKN | Km | This study |
Pc3C | Petroselinum crispum PAL expressed from Ptrc RiboJ in pEEK3C | Km | This study |
At3C | Arabidopsis thaliana PAL expressed from Ptrc RiboJ in pEEK3C | Km | This study |
Np3C | Nostoc punctiforme PAL expressed from Ptrc RiboJ in pEEK3C | Km | This study |
Av3C | Anabaena variabilis PAL expressed from Ptrc RiboJ in pEEK3C | Km | This study |
Ts3C | Treponema socranskii PAL expressed from Ptrc RiboJ in pEEK3C | Km | This study |
Ts3C-H87L | Treponema socranskii PAL with mutation H87L expressed from Ptrc RiboJ in pEEK3C | Km | This study |
Pc3N | Petroselinum crispum PAL expressed from Ptrc RiboJ in pEEK3 | Km | This study |
At3N | Arabidopsis thaliana PAL expressed from Ptrc RiboJ in pEEK3 | Km | This study |
Np3N | Nostoc punctiforme PAL expressed from Ptrc RiboJ in pEEK3 | Km | This study |
Av3N | Anabaena variabilis PAL expressed from Ptrc RiboJ in pEEK3 | Km | This study |
Ts3N | Treponema socranskii PAL expressed from Ptrc RiboJ in pEEK3 | Km | This study |
Ts3N-H87L | Treponema socranskii PAL with mutation H87L expressed from Ptrc RiboJ in pEEK3 | Km | This study |
EvCΔslr1573 | Control strain of Synechocystis WT harboring pEEK* plasmid for Km resistance with knocked-out slr1573 | Km, Cm | This study |
AtCΔslr1573 | Arabidopsis thaliana PAL expressed from Ptrc RBS* in pEEK with knocked-out slr1573 | Km, Cm | This study |
AtNΔslr1573 | Arabidopsis thaliana PAL expressed from Ptrc RBS* in pEEKN with knocked-out slr1573 | Km, Cm | This study |
TsCΔslr1573 | Treponema socranskii PAL expressed from Ptrc RBS* in pEEK with knocked-out slr1573 | Km, Cm | This study |
TsNΔslr1573 | Treponema socranskii PAL expressed from Ptrc RBS* in pEEKN with knocked-out slr1573 | Km, Cm | This study |