Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Expression of phenylalanine ammonia lyases in Synechocystis sp. PCC 6803 and subsequent improvements of sustainable production of phenylpropanoids

Fig. 3

Productivity and Western Blot of Synechocystis strains overexpressing PAL or TAL from the pEEK vector. A tCA production in strains EvC, PcC, PcN, AtC, AtN, NpC, NpN, AvC, AvN, TsC-H87L and TsN-H87L. B pCou production in strains TsC and TsN. Samples of growth medium were taken on day one, three and six. The values are the means of three biological replicates and two technical replicates, error bars represent the standard deviation. A and B are plotted using data from Table 2. C Western Blot and SDS-PAGE of extracts from engineered Synechocystis strains (EvC, PcC, PcN, AtC, AtN, NpC, NpN, AvC, AvN, TsC-H87L, TsN-H87L, TsC and TsN) analyzed for presence of PAL proteins. Upper panel displays the Western Blot using anti-Strep antibody; bottom panel shows the SDS-PAGE loaded with 5 µg of soluble fraction from protein crude extract of the different strains

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us