Fig. 3
From: Biosynthesis and metabolic engineering of 1-hydroxyphenazine in Pseudomonas chlororaphis H18
The protein expression of PhzS and enzymatic catalytic reactions in vitro. A Schematic representation of PCA oxidative decarboxylation by PhzS. B SDS-PAGE of PhzS (43 kD). M, marker; 1, unbound proteins; 2, washed proteins with 20 mM of imidazole; and 3, eluted proteins with 300 mM of imidazole. C HPLC chromatograms of PhzS catalyzed reaction with PCA as substrate at 250 nm. Reaction conditions: phenazine-1-carboxylic acid (0.5 mM), NADH (50 μM), PhzS whole-cell in 50 mM HEPES (pH 7.5) at 30 °C for 1 h. (i) phenazine-1-carboxylic acid standard; (ii) Boiled PhzS whole-cell and NADH with phenazine-1-carboxylic acid; (iii) PhzS whole-cell and NADH with phenazine-1-carboxylic acid; (iv) 1-hydroxyphenazine standard. 1, phenazine-1-carboxylic acid; 4, 1-hydroxyphenazine