Statistical modeling of methylene blue degradation by yeast-bacteria consortium; optimization via agro-industrial waste, immobilization and application in real effluents

Table 5 Estimated effect, regression coefficients and corresponding T and P values in addition to ANOVA analysis for the optimization of MB degradation using CCD

Term	Coef	SE Coef	T	p
Constant	5.2702	0.454	11.608	0
NaNO₃ (gm/L)	− 0.9908	0.2324	− 4.264	0.001
Molasses (μL/L)	2.8167	0.2324	12.122	0
MB (gm)	− 7.205	0.2324	− 31.008	0
Inoculum size (%)	2.5083	0.2324	10.795	0
pH	7.7058	0.2324	33.164	0
(NaNO₃)²	1.356	0.2102	6.452	0
(Molasses)²	1.5985	0.2102	7.606	0
(MB)²	4.0185	0.2102	19.12	0
Inoculum size (%)	2.0423	0.2102	9.717	0
(pH)²	2.3198	0.2102	11.037	0
NaNO₃* Molasses	0.3263	0.2846	1.146	0.276
NaNO₃* MB	4.9438	0.2846	17.372	0
NaNO₃* Inoculum size	− 3.0425	0.2846	− 10.691	0
NaNO₃* pH	− 1.8862	0.2846	− 6.628	0
Molasses * MB	− 3.21	0.2846	− 11.28	0
Molasses * Inoculum size	4.2963	0.2846	15.097	0
Molasses * pH	4.05	0.2846	14.232	0
MB * Inoculum size	− 3.4163	0.2846	− 12.005	0
MB * pH	− 1.41	0.2846	− 4.955	0
Inoculum size * pH	− 2.9688	0.2846	− 10.432	0

Source	Df	Seq SS	Adj SS	Adj MS	F	p
Regression	20	5422.02	5422.02	271.101	209.22	0
Linear	5	3035.98	3035.98	607.195	468.61	0
Square	5	706.06	706.06	141.213	108.98	0
Interaction	10	1679.98	1679.98	167.998	129.65	0
Residual Error	11	14.25	14.25	1.296
Lack of fit	6	11.59	11.59	1.932	3.63	0.089
Pure error	5	2.66	2.66	0.532
Total	31	5436.27

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