Table 1 PBD matrix of independent variables and their concentration with MB degradation as response
Run order | NaCl | MgSO4 | KH2PO4 | K2HPO4 | NaNO3 | MB | Molasses | Inoculum Size (CFU/mL) | Incubation Time (days) | PH | Experimental MB degradation | Predicted MB degradation |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
(gm/L) | (μL) | % | ||||||||||
1 | 1 | 0.63 | 0.45 | 1.9 | 0.21 | 0.5 | 300 | 106 | 3 | 5 | 5.7 | 5.533 |
2 | 1 | 0.21 | 0.15 | 1.9 | 0.63 | 0.3 | 300 | 1012 | 3 | 5 | 37.7 | 37.867 |
3 | 3 | 0.21 | 0.45 | 0.65 | 0.21 | 0.5 | 100 | 1012 | 3 | 5 | 3.8 | 3.967 |
4 | 1 | 0.63 | 0.15 | 0.65 | 0.21 | 0.5 | 300 | 1012 | 1 | 9 | 2.3 | 2.467 |
5 | 3 | 0.63 | 0.15 | 1.9 | 0.63 | 0.5 | 100 | 106 | 1 | 5 | 1.7 | 1.867 |
6 | 3 | 0.21 | 0.45 | 1.9 | 0.21 | 0.3 | 300 | 106 | 1 | 5 | 6.6 | 6.767 |
7 | 1 | 0.63 | 0.45 | 0.65 | 0.63 | 0.3 | 100 | 106 | 3 | 9 | 4.4 | 4.567 |
8 | 3 | 0.63 | 0.15 | 1.9 | 0.21 | 0.3 | 100 | 1012 | 3 | 9 | 7.3 | 7.133 |
9 | 3 | 0.63 | 0.45 | 0.65 | 0.63 | 0.3 | 300 | 1012 | 1 | 5 | 23.7 | 23.533 |
10 | 3 | 0.21 | 0.15 | 0.65 | 0.63 | 0.5 | 300 | 106 | 3 | 9 | 5.1 | 4.933 |
11 | 1 | 0.21 | 0.45 | 1.9 | 0.63 | 0.5 | 100 | 1012 | 1 | 9 | 2.3 | 2.133 |
12 | 1 | 0.21 | 0.15 | 0.65 | 0.21 | 0.3 | 100 | 106 | 1 | 5 | 14.4 | 14.233 |