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Fig. 7 | Microbial Cell Factories

Fig. 7

From: PglB function and glycosylation efficiency is temperature dependent when the pgl locus is integrated in the Escherichia coli chromosome

Fig. 7

In vitro glycosylation reaction using carrier protein G-FlpA. L-Protein ladder PageRuler plus (Bioline, UK) Lane 1, 4 and 7—negative control, G-FlpA, no glycan donor incubated at 30 °C, 37 °C and 42 °C respectively; lane 2,5 and 8—G-FlpA combined with glycan donor from SDB1pACYCpgl and SDB1pEXT21pglB, incubated at 30 °C, 37 °C and 42 °C respectively; lane 3, 6 and 9—G-FlpA combined with glycan donor from SDB1pgl and SDB1pEXT21pglB incubated at 30 °C, 37 °C and 42 °C respectively

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Microbial Cell Factories

ISSN: 1475-2859

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