Table 2 Plasmid and yeast strains used in this study
Plasmids/strains | Genotype | Source |
|---|---|---|
Plasmids | ||
pUMRI-21 | loxp-KanMX-URA3-pbr322ori-loxp, TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1, No Homologous Arms | Lv et al. [31] (KM216411) |
pBX-11 | loxp-KanMX-URA3-pbr322ori-loxp, ΔDGA1: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-12 | loxp-KanMX-URA3-pbr322ori-loxp, ΔLRO1: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-13 | loxp-KanMX-URA3-pbr322ori-loxp, ΔARE1: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-14 | loxp-KanMX-URA3-pbr322ori-loxp, ΔARE2: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-15 | loxp-KanMX-URA3-pbr322ori-loxp, ΔTGL3: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-16 | loxp-KanMX-URA3-pbr322ori-loxp, ΔTGL4: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-17 | loxp-KanMX-URA3-pbr322ori-loxp, ΔTGL5: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-18 | loxp-KanMX-URA3-pbr322ori-loxp, ΔFLD1: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-19 | loxp-KanMX-URA3-pbr322ori-loxp, ΔHO: TADH1-MCS1-PGAL10-PGAL1-MCS2-TCYC1 | This study |
pBX-20 | loxp-KanMX-URA3-pbr322ori-loxp, ΔHO: TADH1-PAH1-PGAL10-PGAL1-DGA1-TCYC1 | This study |
pBX-21 | loxp-KanMX-URA3-pbr322ori-loxp, PTEF1-ACC1 S659A, S1157A -TCYC1 | This study |
pBX-22 | loxp-KanMX-URA3-pbr322ori-loxp, ΔTy4: PTEF1-ACC1 S659A, S1157A -TCYC1 | This study |
pBX-OLE1pro | ΔPERG9::POLE1 | This study |
pBX-MGA2pro | ΔPERG9::PMGA2 | This study |
pBX-IZH1pro | ΔPERG9::PIZH1 | This study |
pBX-IZH4pro | ΔPERG9::PIZH4 | This study |
pBX-YDR274Cpro | ΔPERG9::PYDR274C | This study |
pBX-23 | loxp-KanMX-URA3-pbr322ori-loxp, TADH1-MCS1-PPYK1-PTPI1-MCS2-TCYC1 | This study |
pBX-24 | Δdpp1::TADH1-CrtYB-PPYK1-PTPI1-CrtI-TCYC1, loxp-KanMX-URA3-pbr322ori-loxp | This study |
pBX-25 | Δlpp1::TADH1-MCS-PPYK1-PTPI1-CrtE-TCYC1, loxp-KanMX-URA3-pbr322ori-loxp | This study |
Strains | ||
YBX-ld1 | YBX-01,Δdga1 | This study |
YBX-ld2 | YBX-ld1,Δlro1 | This study |
YBX-ld3 | YBX-ld2, Δare1 | This study |
YBX-ld4 | YBX-ld3, Δare2 | This study |
YBX-22 | YBX-01,Δho::TADH1-DGA1-PGAL10-PGAL1-PAH1-TCYC1; ΔTy4::PTEF1-ACC1S659A, S1157A-TCYC1 | This study |
YBX-23 | YBX-22, Δtgl3, Δtgl4, Δtgl5 | This study |
YBX-24 | YBX-23, Δfld1 | This study |
YBX-01-OLE1 | YBX-01, ΔPERG9::POLE1 | This study |
YBX-01-MGA2 | YBX-01, ΔPERG9::PMGA2 | This study |
YBX-01-IZH1 | YBX-01, ΔPERG9::PIZH1 | This study |
YBX-01-IZH4 | YBX-01, ΔPERG9::PIZH4 | This study |
YBX-01-YDR274C | YBX-01, ΔPERG9::PYDR274C | This study |
YBX-41 | YBX-01-IZH1, Δdpp1::TADH1-CrtYB-PPYK1-PTPI1-CrtI-TCYC1, Δlpp1::PTPI1-CrtE-TCYC1 | This study |