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Table 1 Relative production purity of M. neoaurum DSM 44074 and its derivative strains

From: Production of 9,21-dihydroxy-20-methyl-pregna-4-en-3-one from phytosterols in Mycobacterium neoaurum by modifying multiple genes and improving the intracellular environment

Strain

Relative purity (%)

9-OH-AD

9-OH-4-HP

AD

Others

DSM 44704

0

0

0

0

ΔkstD

90.4 ± 3.1

5.6 ± 2.5

1.9 ± 0.2

2.1 ± 0.8

ΔkstDΔhsd4A

7.2 ± 2.2

88.6 ± 1.3

2.4 ± 0.5

1.8 ± 1.3

ΔkstDΔfadA5

8.0 ± 1.9

86.0 ± 3.5

3.3 ± 0.4

2.7 ± 0.5

ΔkstDΔhsd4A-hsd4A

90.0 ± 2.2

5.2 ± 1.7

1.8 ± 0.2

3.0 ± 0.3

ΔkstDΔfadA5-fadA5

88.5 ± 3.6

4.4 ± 2.3

1.3 ± 0.1

5.8 ± 1.2

ΔkstDΔhsd4AΔfadA5

2.0 ± 1.1

94.9 ± 1.2

0.3 ± 0.1

2.8 ± 0.3

ΔkstDΔhsd4AΔfadA5-NK

2.0 ± 1.1

97.0 ± 1.1

0.3 ± 0.1

0.7 ± 0.3