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Table 1 Plasmids used in this study

From: Influence of prefoldin subunit 4 on the tolerance of Kluyveromyces marxianus to lignocellulosic biomass-derived inhibitors

Plasmids

Description

References

YEGAP

AmpR, ScTRP1, PScGAPDH, TScGAPDH

[37]

YEUGAP

AmpR, ScURA3, PScGAPDH, TScGAPDH

[17]

YEUKmPGK

AmpR, ScURA3, PKmPGK, TScGAPDH

[38]

pGEM-T Easy

AmpR

Promega

pGEM-T-â–³ScURA3

AmpR, nonfunctional ScURA3

[18]

pPCG

ZeocinR, CBM-EGFP- pPICZ αA

[39]

pSY001

AmpR, KmPFD4-pGEM-T Easy vector

AmpR, KmPFD4-T vector

This study

pSY002

AmpR, KmPFD4 inserted with ScURA3

This study

pSY003

AmpR, ScTRP1, PScGAPDH- KmPFD4 -TScGAPDH

This study

pSY004

AmpR, ScURA3, PScGAPDH-EGFP- TScGAPDH

This study

pSY005

AmpR, ScURA3, PScGAPDH- KmPFD4 -EGFP-TScGAPDH

This study