High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741T and in silico analyses of the genus specific PhaC2 polymerase variant

Table 1 PHA productivity for Cobetia sp. MC34 and C. marina DSM 4741^T

Strain	Carbon substrate	OD₆₀₀ at harvest	CDW (g/L)	PHB (% of CDW)	PHV (% of CDW)	PHA (g/L)
Cobetia sp. MC34	Acetate	15.0 (± 1.3)	3.4 (± 0.5)	72 (± 11)	–	2.5
	Acetate + valerate	14.2 (± 2.1)	3.4 (± 0.8)	48 (± 3.4)	14 (± 2.3)	2.1
	Glycerol	6.2 (± 1.4)	2.5 (± 0.5)	26 (± 12)	–	0.7
	Glucose	6.5 (± 0.3)	1.8 (± 0.1)	35 (± 4.8)	–	0.6
	Fructose	6.0 (± 0.8)	3.9 (± 0.8)	9.9 (± 1.3)	–	0.4
Cobetia marina DSM 4741^T	Acetate	15.0 (± 2.9)	3.9 (± 0.6)	61 (± 8.3)	–	2.4
	Acetate + valerate	17.0 (± 1.4)	4.4 (± 0.3)	59 (± 11)	26 (± 2.6)	3.7
	Glycerol	14.0 (± 2.1)	4.0 (± 0.1)	61 (± 8.1)	–	2.5
	Glucose	8.0 (± 0.2)	2.4 (± 0.3)	46 (± 2.9)	–	1.1
	Fructose	8.1 (± 0.2)	3.1 (± 0.2)	28 (± 11)	–	0.9

The cells were harvested from 80 mL cultures in MMCY_2 medium supplemented with 2% sodium acetate with- and without 8 mM sodium valerate, with 1.5% glycerol, 4.0% glucose or 4.0% fructose and were quantified by GC-FID analysis (n = 6). All Cobetia sp. MC34 cultures were grown for 32 h. The C. marina DSM 4741^T cultures supplemented with glucose and fructose were grown for 24 h, and those supplemented with acetate, valerate and glycerol were grown for 28 h. Values in parentheses are standard deviations calculated from 6 samples.

ISSN: 1475-2859