Fig. 2

Putative PHA production on MMY agar plates supplemented with an excess carbon substrate. A Fluorescent microscopy of Nile-red stained cells grown on MMY agar plates supplemented with 2% glucose and 2% glycerol shows accumulation of intracellular inclusions after 72 h incubation at 14 °C, in contrast to the negative controls. The cells were imaged in a ZEISS Axio Observer 7 microscope at 100X magnification and the fluorescence intensity normalized to 1500 for all images. The scale bar shows 2 μm. B FT-IR spectra of cells from the same agar plates as in A. The cells grown on glucose and glycerol supplemented plates show carbonyl-ester signals centered at wavenumber 1724 cm^–1 (Green box, n = 2). The amide band I-ester from proteins is observed in spectra from all plates, including the negative controls (Orange box, n = 2).