Table 1 Summarized Donor Screening Recommendations

Inclusion Criteria

^a40 ≥ Age ≥ 18

Body mass index (< 28 or > 18.5 kg/m²)

Providing informed consent

Keeping honesty and self-discipline

Feeling well at the period of donation

Children may donate with parental consent and child’s assent

Exclusion Criteria

High-risk behaviors

   Sexual practices associated with high risk of acquiring infectious diseases in last 12 months

   Known exposure with HIV, HAV, HBV, HCV infection in the last 12 months

   Intravenous drug use, incarceration, tattoo, piercing within previous 6 months

   Risk factors for variant Cruetzfeldt-Jakob disease

Communicable disease

   History of HIV, HAV, HBV or HCV infection

   Any of the following in the previous 4 weeks: fever, vomiting, diarrhea or other symptoms of infection

   Any of the following in the previous 8 weeks: vaccinations, injections or contact with a recipient of the smallpox vaccine

   Any of the following in the previous 12 months: blood transfusion, accidental needle stick or blood exposure

   Travel within previous 6 months to areas of high risk of travelers’ diarrhea

   Close contacts with active gastrointestinal infection

General medical illness or use of medication

   Social history (e.g., smoking, drinking, etc.)

   Receipt of antibiotics or PPI in the previous 3 months

   Using of medications (e.g., an experimental medicine, immunomodulatory therapy, chemotherapy, etc.)

   History of intrinsic gastrointestinal disease (e.g., inflammatory bowel disease, irritable bowel syndrome, chronic constipation, gastrointestinal malignancy, prior major gastrointestinal surgery, procedure, etc.)

   Strong family history of colorectal cancer

   Disease history (e.g., malignancy, malnutrition, chronic pain syndromes, neurologic or neurodevelopmental disorders, autoimmune or atopic illness, cardiovascular/metabolic disease, diabetes, hypertension, stroke, mental diseases, etc.)

   ^aOral diseases (Caries, periodontal diseases, mucosal diseases or oral cancer)

Others

   ^aHamilton Anxiety Rating Scale (> 7 score), Hamilton Depression Rating Scale (> 7 score)

   ^aLifestyle questionnaire survey (almost never exercise)

   Logistics issue (e.g., unable to donate regularly, distance to donor facility)

   Restrictive diet (e.g., gluten free diet)

   Abnormal vital sign (e.g., unexplained syncope)

Gastrointestinal pathogens testing

Routine tests

   Stool microscopy and culture, fecal occult blood test

Intestinal parasites

   Fecal egg, cyst, microsporidia and parasites, Blastocystis hominis, Strongyloides stercoralis, Cyclospora, Isospora, Giardia, Cryptosporidium

Intestinal pathogenic virus

   Rotavirus, Norovirus, Adenovirus

Gastrointestinal pathogenic bacteria

   Helicobacter pylori, C. difficile, Entamoeba, Shiga toxin with reflex to O157, Salmonella/Shigella, Vibrio cholera O1 and O139, Listeria monocytogenes, Escherichia coli O157 H7, Yersinia, Campylobacter

Intestinal drug-resistance bacteria

   Methicillin-Resistant Staphylococcus aureus, Vancomycin-Resistant Enterococci, Carbapenem-Resistant Enterobacteriaceae, Extended-Spectrum β-Lactamases bacteria

Blood testing

Routine tests

   Complete blood count, Liver function test, Renal function test, Blood glucose, Elevated C-Reactive Protein levels, Elevated dynamic ESR

   ^aLipidemia test

Infectious pathogen

   Treponema pallidum serology

   HTLV I/II, HAV, HBV, HCV, HEV, HIV

   ^aSARS-CoV-2 virus